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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
25/09/2016 |
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Actualizado : |
09/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
ARRUABARRENA, A.; BENITEZ-GALEANO, M.J.; GIAMBIASI, M.; BERTALMIO, A.; COLINA, R.; HERNÁNDEZ-RODRÍGUEZ, L. |
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Afiliación : |
ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA JOSÉ BENÍTEZ-GALEANO, Laboratorio de Virología Molecular, Centro Universitario Regional Noroeste (CENUR Noroeste), Universidad de la República; MARIO ALEJANDRO GIAMBIASI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA MARIA BERTALMIO CASARIEGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODNEY COLINA, Laboratorio de Virología Molecular, Centro Universitario Regional Noroeste (CENUR Noroeste), Universidad de la República; LESTER HERNÁNDEZ-RODRÍGUEZ, Instituto de Investigaciones en Fruticultura Tropical, La Habana, Cuba. |
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Título : |
Application of a simple and affordable protocol for isolating plant total nucleic acids for RNA and DNA virus detection. |
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Fecha de publicación : |
2016 |
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Fuente / Imprenta : |
Journal of Virological Methods, 2016, v.237, p. 14-17. |
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DOI : |
10.1016/j.jviromet.2016.08.011 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 30 May 2016 / Received in revised form 26 July 2016 / Accepted 14 August 2016 / Available online 16 August 2016. |
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Contenido : |
ABSTRACT.
Standard molecular methods for plant virus diagnosis require the purification of RNA or DNA extracts from a large number of samples, with sufficient concentration and quality for their use in PCR, RT-PCR, or qPCR analysis. Most methods are laborious and use either hazardous and/or costly chemicals.Apreviously published protocol for RNA isolation from several plant species yields high amounts of good quality RNADNA mixture in a simple, safe and inexpensive manner. In the present work, this method was tested to obtain RNA-DNA extracts from leaves of tomato, potato and three species of citrus, and was compared with two commercial kits. The results demonstrated that this protocol offers at least comparable nucleic
acid quality, quantity and purity to those provided by commercial phenol-based or spin column systems and that are suitable to be used in PCR, RT-PCR and qPCR for virus and viroid detection. Because of its easy implementation and the use of safe and inexpensive reagents, it can be easily implemented to work in plant virus and viroid detection in different plant species.
© 2016 Elsevier B.V. All rights reserved |
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Palabras claves : |
DNA; PCR; PLANT VIROID; PLANT VIRUS; PURIFICATION; RNA; RT-PCR. |
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Thesagro : |
CITRUS. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02137naa a2200301 a 4500
001 1055729
005 2019-10-09
008 2016 bl uuuu u00u1 u #d
024 7 $a10.1016/j.jviromet.2016.08.011$2DOI
100 1 $aARRUABARRENA, A.
245 $aApplication of a simple and affordable protocol for isolating plant total nucleic acids for RNA and DNA virus detection.$h[electronic resource]
260 $c2016
500 $aArticle history: Received 30 May 2016 / Received in revised form 26 July 2016 / Accepted 14 August 2016 / Available online 16 August 2016.
520 $aABSTRACT. Standard molecular methods for plant virus diagnosis require the purification of RNA or DNA extracts from a large number of samples, with sufficient concentration and quality for their use in PCR, RT-PCR, or qPCR analysis. Most methods are laborious and use either hazardous and/or costly chemicals.Apreviously published protocol for RNA isolation from several plant species yields high amounts of good quality RNADNA mixture in a simple, safe and inexpensive manner. In the present work, this method was tested to obtain RNA-DNA extracts from leaves of tomato, potato and three species of citrus, and was compared with two commercial kits. The results demonstrated that this protocol offers at least comparable nucleic acid quality, quantity and purity to those provided by commercial phenol-based or spin column systems and that are suitable to be used in PCR, RT-PCR and qPCR for virus and viroid detection. Because of its easy implementation and the use of safe and inexpensive reagents, it can be easily implemented to work in plant virus and viroid detection in different plant species. © 2016 Elsevier B.V. All rights reserved
650 $aCITRUS
653 $aDNA
653 $aPCR
653 $aPLANT VIROID
653 $aPLANT VIRUS
653 $aPURIFICATION
653 $aRNA
653 $aRT-PCR
700 1 $aBENITEZ-GALEANO, M.J.
700 1 $aGIAMBIASI, M.
700 1 $aBERTALMIO, A.
700 1 $aCOLINA, R.
700 1 $aHERNÁNDEZ-RODRÍGUEZ, L.
773 $tJournal of Virological Methods, 2016$gv.237, p. 14-17.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
16/10/2014 |
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Actualizado : |
20/09/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CUBBAGE, F.; BALMELLI, G.; BUSSONI, A.; NOELLEMEYER, E.; PACHAS, A.N.; FASSOLA, H.; COLCOMBET, L.; ROSSNER, B.; FREY, G.; DUBE, F.; LOPES DE SILVA, M.; STEVENSON, H.; HAMILTON, J.; HUBBARD, W. |
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Afiliación : |
FREDERICK CUBBAGE; GUSTAVO DANIEL BALMELLI HERNANDEZ, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; ADRIANA BUSSONI; ELKE NOELLEMEYER; ANIBAL N. PACHAS; HUGO FASSOLA; LUIS COLCOMBET; BELÉN ROSSNER; GREGORY FREY; FRANCIS DUBE; MARCIO LOPES DE SILVA; HAYLEY STEVENSON; JAMES HAMILTON; WILLIAM HUBBARD. |
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Título : |
Comparing silvopastoral systems and prospects in eight regions of the world. |
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Fecha de publicación : |
2012 |
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Fuente / Imprenta : |
Agroforest Systems, 2012, v. 86, p. 303-314 |
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DOI : |
10.1007/s10457-012-9482-z |
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Idioma : |
Inglés |
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Notas : |
History article: Received: 20 October 2011; Accepted: 6 January 2012; Published online: 5 February 2012. |
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Contenido : |
Silvopasture systems combine trees, forage, and livestock in a variety of different species and management regimes, depending on the biophysical, economic, cultural, and market factors in a region. We describe and compare actual farm practices and current research trials of silvopastoral systems in eight regions within seven countries of the world: Misiones and Corrientes provinces, Argentina; La Pampa province, Argentina; northwestern Minas Gerais, Brazil; the Ayse´n region of Patagonia, Chile; the North Island of New Zealand; the Southeast United States; Paraguay; and Uruguay. Some countries use native trees and existing forests; some use plantations, particularly of exotic species. Natural forest silvopasture systems generally add livestock in extensive systems, to capture the benefits of shade, forage, and income
diversification without much added inputs. Plantation forest systems are more purposive and intensive, with more focus on joint production and profits, for small
owners, large ranches, and timber companies. Trends suggest that more active management of both natural and planted silvopastoral systems will be required to
enhance joint production of timber and livestock, achieve income diversification and reduce financial risk, makemore profit, improve environmental benefits,
and realize more resilience to adapt to climate change. |
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Palabras claves : |
ADOPTION; NEW ZEALAND; SILVOPASTORAL SYSTEMS; SILVOPASTURE; SOUTH AMERICA; USA. |
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Thesagro : |
SILVOPASTOREO. |
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Asunto categoría : |
A50 Investigación agraria |
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Marc : |
LEADER 02447naa a2200385 a 4500
001 1051153
005 2019-09-20
008 2012 bl uuuu u00u1 u #d
024 7 $a10.1007/s10457-012-9482-z$2DOI
100 1 $aCUBBAGE, F.
245 $aComparing silvopastoral systems and prospects in eight regions of the world.
260 $c2012
500 $aHistory article: Received: 20 October 2011; Accepted: 6 January 2012; Published online: 5 February 2012.
520 $aSilvopasture systems combine trees, forage, and livestock in a variety of different species and management regimes, depending on the biophysical, economic, cultural, and market factors in a region. We describe and compare actual farm practices and current research trials of silvopastoral systems in eight regions within seven countries of the world: Misiones and Corrientes provinces, Argentina; La Pampa province, Argentina; northwestern Minas Gerais, Brazil; the Ayse´n region of Patagonia, Chile; the North Island of New Zealand; the Southeast United States; Paraguay; and Uruguay. Some countries use native trees and existing forests; some use plantations, particularly of exotic species. Natural forest silvopasture systems generally add livestock in extensive systems, to capture the benefits of shade, forage, and income diversification without much added inputs. Plantation forest systems are more purposive and intensive, with more focus on joint production and profits, for small owners, large ranches, and timber companies. Trends suggest that more active management of both natural and planted silvopastoral systems will be required to enhance joint production of timber and livestock, achieve income diversification and reduce financial risk, makemore profit, improve environmental benefits, and realize more resilience to adapt to climate change.
650 $aSILVOPASTOREO
653 $aADOPTION
653 $aNEW ZEALAND
653 $aSILVOPASTORAL SYSTEMS
653 $aSILVOPASTURE
653 $aSOUTH AMERICA
653 $aUSA
700 1 $aBALMELLI, G.
700 1 $aBUSSONI, A.
700 1 $aNOELLEMEYER, E.
700 1 $aPACHAS, A.N.
700 1 $aFASSOLA, H.
700 1 $aCOLCOMBET, L.
700 1 $aROSSNER, B.
700 1 $aFREY, G.
700 1 $aDUBE, F.
700 1 $aLOPES DE SILVA, M.
700 1 $aSTEVENSON, H.
700 1 $aHAMILTON, J.
700 1 $aHUBBARD, W.
773 $tAgroforest Systems, 2012$gv. 86, p. 303-314
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